Plasmid_Backbone
pBS1C_Cot

Part:BBa_K3653015:Design

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-12)


pBS1C_CotG_Sybody15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 932
    Illegal NheI site found at 1064
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 932
    Illegal BglII site found at 1049
    Illegal BglII site found at 6331
    Illegal XhoI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 932
    Illegal XbaI site found at 16
    Illegal NgoMIV site found at 2470
    Illegal NgoMIV site found at 4796
    Illegal NgoMIV site found at 6706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2646
    Illegal SapI.rc site found at 3728


Design Notes

No


Source

The plasmid: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177231/ The Cot: https://link.springer.com/article/10.1186/1475-2859-13-30

References