Plasmid_Backbone
pBS1C_Cot
Part:BBa_K3653015:Design
Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker Group: iGEM20_Technion-Israel (2020-10-12)
pBS1C_CotG_Sybody15
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 1
Plasmid lacks a suffix.
Illegal EcoRI site found at 35
Illegal EcoRI site found at 932 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal EcoRI site found at 35
Illegal EcoRI site found at 932
Illegal NheI site found at 1064
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal EcoRI site found at 35
Illegal EcoRI site found at 932
Illegal BglII site found at 1049
Illegal BglII site found at 6331
Illegal XhoI site found at 670 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 1
Plasmid lacks a suffix.
Illegal EcoRI site found at 35
Illegal EcoRI site found at 932 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1
Plasmid lacks a suffix.
Illegal EcoRI site found at 35
Illegal EcoRI site found at 932
Illegal XbaI site found at 16
Illegal NgoMIV site found at 2470
Illegal NgoMIV site found at 4796
Illegal NgoMIV site found at 6706 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2646
Illegal SapI.rc site found at 3728
Design Notes
No
Source
The plasmid: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177231/ The Cot: https://link.springer.com/article/10.1186/1475-2859-13-30